Itoi T, Ohyashiki K, Yahata N, Shinohara Y, Takei K, Takeda K, Nagao K, Hisatomi H, Ebihara Y, Shay J W, Saito T
Fourth Department of Internal Medicine, Tokyo Medical University, Shinjuku-ku, Tokyo 160-0023, Japan.
Int J Oncol. 1999 Dec;15(6):1061-7. doi: 10.3892/ijo.15.6.1061.
Telomerase is detected by the telomeric repeat amplification protocol (TRAP) assay in more than 85% of primary cancers. In the present study, we determined telomerase activity using exfoliated bile cells obtained from biliary tract neoplasia specimens. The aim of this study was to provide additional information regarding minimally invasive approaches to the detection of biliary tract cancer in combination with routine cytologic examination. We analyzed for telomerase activity bile juice from patients with gallbladder carcinoma, cholangiocarcinoma, cholecystitis and cholangitis. Semiquantitative determination of telomerase activity was performed using both a fluorescence-based TRAP assay on cell extracts and at the cellular level by an in situ TRAP assay. The fluorescence-based TRAP assay detected bile telomerase activity in samples from 4 of 10 patients with biliary tract cancer. In contrast, the in situ TRAP assay detected telomerase positive cells in samples from 6 of 10 patients with biliary tract cancer. However, only one of these samples showed class V cytology. A combination of semiquantitative analysis and an in situ TRAP assay to detect telomerase positive cells may improve the diagnosis of biliary tract cancers with the combination of routine cytologic examination.
在超过85%的原发性癌症中，通过端粒重复序列扩增法（TRAP）检测到端粒酶。在本研究中，我们使用从胆道肿瘤标本中获取的脱落胆汁细胞测定端粒酶活性。本研究的目的是结合常规细胞学检查，提供有关检测胆道癌的微创方法的更多信息。我们分析了胆囊癌、胆管癌、胆囊炎和胆管炎患者的胆汁中的端粒酶活性。使用基于荧光的TRAP分析法对细胞提取物进行端粒酶活性的半定量测定，并通过原位TRAP分析法在细胞水平进行测定。基于荧光的TRAP分析法在10例胆道癌患者中的4例样本中检测到胆汁端粒酶活性。相比之下，原位TRAP分析法在10例胆道癌患者中的6例样本中检测到端粒酶阳性细胞。然而，这些样本中只有一个显示为V级细胞学。结合常规细胞学检查，采用半定量分析和原位TRAP分析法检测端粒酶阳性细胞可能会改善胆道癌的诊断。
