拟南芥分生组织层1启动子决定了分生组织和幼嫩原基中的表皮表达。

The Arabidopsis thaliana MERISTEM LAYER 1 promoter specifies epidermal expression in meristems and young primordia.

作者信息

Sessions A, Weigel D, Yanofsky M F

机构信息

Department of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA.

出版信息

Plant J. 1999 Oct;20(2):259-63. doi: 10.1046/j.1365-313x.1999.00594.x.

DOI:10.1046/j.1365-313x.1999.00594.x

PMID:10571886

Abstract

The Arabidopsis thaliana MERISTEM LAYER 1 (ATML1) gene is expressed in the epidermis of developing embryos and shoot meristems. To identify regulatory sequences necessary for epidermis-specific expression, three fusions of overlapping ATML1 genomic sequences to the GUS reporter gene were introduced into Arabidopsis plants. All fusion genes conferred epidermis-specific expression of both GUS mRNA and protein activity but varied in both the timing and relative levels of expression, suggesting partial redundancy of ATML1 regulatory elements. This study defines L1-specific regulatory sequences that are sufficient to direct foreign gene expression in a layer-specific manner.

摘要

拟南芥分生组织层1（ATML1）基因在发育中的胚胎和茎尖分生组织的表皮中表达。为了鉴定表皮特异性表达所必需的调控序列，将重叠的ATML1基因组序列与GUS报告基因的三种融合体导入拟南芥植株中。所有融合基因都赋予了GUS mRNA和蛋白质活性的表皮特异性表达，但在表达的时间和相对水平上有所不同，这表明ATML1调控元件存在部分冗余。本研究定义了L1特异性调控序列，这些序列足以以层特异性方式指导外源基因表达。