大肠杆菌在细胞中心含有一个DNA复制区室。

Escherichia coli contains a DNA replication compartment in the cell center.

作者信息

Koppes L J, Woldringh C L, Nanninga N

机构信息

Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, the Netherlands.

出版信息

Biochimie. 1999 Aug-Sep;81(8-9):803-10. doi: 10.1016/s0300-9084(99)00217-5.

DOI:10.1016/s0300-9084(99)00217-5

PMID:10572292

Abstract

The active replication forks of E. coli B/r K cells growing with a doubling time of 210 min have been pulse-labeled with [(3)H] thymidine for 10 min. By electron-microscopic autoradiography the silver grains have been localized in the various length classes. From the known pattern of the DNA replication period in the cell cycle at slow growth and from the average position of grains per length class it was deduced that DNA replication starts in the cell center and that it remains there for a substantial part of the DNA replication period. This suggests the occurrence of a centrally located DNA replication compartment.

摘要

以210分钟的倍增时间生长的大肠杆菌B/r K细胞的活跃复制叉，用[³H]胸苷脉冲标记10分钟。通过电子显微镜放射自显影，已将银颗粒定位在不同长度类别中。根据细胞周期中慢生长时DNA复制期的已知模式以及每个长度类别中颗粒的平均位置，推断出DNA复制始于细胞中心，并且在DNA复制期的大部分时间内都保持在那里。这表明存在一个位于中心的DNA复制区室。