肌动蛋白同源物MreB与RNA聚合酶相互作用，二者都是大肠杆菌染色体分离所必需的。
Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli.
作者信息
Kruse Thomas, Blagoev Blagoy, Løbner-Olesen Anders, Wachi Masaaki, Sasaki Kumi, Iwai Noritaka, Mann Matthias, Gerdes Kenn
机构信息
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK-5230 Odense M, Denmark.
出版信息
Genes Dev. 2006 Jan 1;20(1):113-24. doi: 10.1101/gad.366606.
Abstract
The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.
摘要
肌动蛋白样MreB细胞骨架蛋白和RNA聚合酶（RNAP）均被认为可为染色体分离提供动力。在此，我们确定MreB和RNAP为体内相互作用伙伴。使用体外纯化的组分证实了这种相互作用。我们还提供了令人信服的证据，表明MreB和RNAP都是大肠杆菌中染色体分离所必需的。MreB是起始点和大量DNA分离所必需的，而RNAP是大量DNA、终点以及可能也是起始点分离所必需的。此外，流式细胞术分析表明，MreB缺失和RNAP失活导致几乎相同且非常异常的染色体分离缺陷。因此，我们的结果提出了MreB-RNAP相互作用在染色体分离中具有功能重要性的可能性。
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