Cho S H, Chung I J, Lee J J, Park M L, Kim H J
Department of Internal Medicine, Chonnam National University Medical School, Kwangju, Korea.
J Korean Med Sci. 1999 Oct;14(5):520-5. doi: 10.3346/jkms.1999.14.5.520.
To compare the clonogenicity and distribution of CD34+ subsets in bone marrow (BM), granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood (PB) and cord blood (CB), we analyzed in vitro colony formation and CD34+ cells co-expressing differentiation molecules (CD38, HLA-DR), myeloid associated molecules (CD13, CD33), a T-cell associated molecule (CD3), and a B-cell associated molecule (CD19) from mononuclear cells (MNCs) in the three compartments. The proportions of CD34+CD38- cells (BM: 4.4+/-2.8%, PB: 5.3+/-2.1%, CB: 5.9+/-3.9%) and CD34+HLA-DR cells (BM: 4.7+/-3.4%, PB: 5.5+/-2.3%, CB: 6.1+/-3.7%) did not differ significantly among the compartments. In contrast, a significantly higher proportion of CD34 cells of PB and CB co-expressed CD13 (75.0+/-11.4%, 77.7+/-17.3%) and CD33 (67.1 +/-5.7%, 56.8+/-10.3%) compared with those of BM (43.0+/-6.3%, 27.6+/-5.1%) and a significantly higher number of granulocyte-macrophage colony-forming units (CFU-GM) and erythroid burst-forming units (BFU-E) were detected in MNCs derived from PB and CB compared with those from BM (p<0.01). The proportion of CD34+CD19+ cells was higher in BM (34.9+/-11.9%) than those in PB (5.6+/-3.0%) and CB (4.7=2.1%) (p<0.05). The proportion of CD34+CD3+ was comparable in all three compartments. In conclusion, our findings show that MNCs of mobilized PB and CB display similar phenotypic profiles of CD34+ subsets and clonogenicity, different from those of BM.
为比较骨髓（BM）、粒细胞集落刺激因子（G-CSF）动员的外周血（PB）和脐血（CB）中CD34+亚群的克隆形成能力和分布，我们分析了三个区室中单个核细胞（MNC）的体外集落形成情况以及共表达分化分子（CD38、HLA-DR）、髓系相关分子（CD13、CD33）、T细胞相关分子（CD3）和B细胞相关分子（CD19）的CD34+细胞。CD34+CD38-细胞（BM：4.4±2.8%，PB：5.3±2.1%，CB：5.9±3.9%）和CD34+HLA-DR细胞（BM：4.7±3.4%，PB：5.5±2.3%，CB：6.1±3.7%）的比例在各区室之间无显著差异。相比之下，与BM（43.0±6.3%，27.6±5.1%）相比，PB和CB中CD34细胞共表达CD13（75.0±11.4%，77.7±17.3%）和CD33（67.1±5.7%，56.8±10.3%）的比例显著更高，并且与来自BM的MNC相比，在来自PB和CB的MNC中检测到显著更多的粒细胞-巨噬细胞集落形成单位（CFU-GM）和红系爆式集落形成单位（BFU-E）（p<0.01）。CD34+CD19+细胞的比例在BM中（34.9±11.9%）高于PB（5.6±3.0%）和CB（4.7±2.1%）（p<0.05）。CD34+CD3+的比例在所有三个区室中相当。总之，我们的研究结果表明，动员的PB和CB的MNC显示出与BM不同的、相似的CD34+亚群表型特征和克隆形成能力。
