一个与合浦珠母贝珍珠层形成相关的新基质蛋白家族。
A new matrix protein family related to the nacreous layer formation of Pinctada fucata.
作者信息
Samata T, Hayashi N, Kono M, Hasegawa K, Horita C, Akera S
机构信息
Laboratory of Cell Biology, Faculty of Environmental Health, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Japan.
出版信息
FEBS Lett. 1999 Nov 26;462(1-2):225-9. doi: 10.1016/s0014-5793(99)01387-3.
We have isolated a new matrix protein family (N16) which is specific to the nacreous layer of the Japanese pearl oyster, Pinctada fucata, and have cloned and characterized the cDNAs coding for the components. Analysis of the deduced amino acid sequence revealed that N16 showed no definitive homology with other proteins. The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation without it. The aragonite crystals showed platy morphology different from those formed inorganically, and long intervals of incubation resulted in crystalline layers highly similar to the nacreous layer.
我们分离出了一种新的基质蛋白家族（N16），它是日本珍珠贝（Pinctada fucata）珍珠层所特有的，并克隆和鉴定了编码这些成分的cDNA。对推导的氨基酸序列分析表明，N16与其他蛋白质没有明确的同源性。结晶的体外研究表明，N16固定在基质上时会诱导文石晶体形成，但没有固定时则会抑制晶体形成。文石晶体呈现出与无机形成的晶体不同的片状形态，长时间孵育会形成与珍珠层高度相似的晶体层。
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