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软体动物 Pif/BMSPs 中的 von Willebrand 因子 A 和几丁质结合结构域的多样化。

Diversification of von Willebrand Factor A and Chitin-Binding Domains in Pif/BMSPs Among Mollusks.

机构信息

Research Institute for Global Change, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-Cho, Yokosuka, Kanagawa, 237-0061, Japan.

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan.

出版信息

J Mol Evol. 2024 Aug;92(4):415-431. doi: 10.1007/s00239-024-10180-1. Epub 2024 Jun 12.

DOI:10.1007/s00239-024-10180-1
PMID:38864871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11291548/
Abstract

Pif is a shell matrix protein (SMP) identified in the nacreous layer of Pinctada fucata (Pfu) comprised two proteins, Pif97 and Pif 80. Pif97 contains a von Willebrand factor A (VWA) and chitin-binding domains, whereas Pif80 can bind calcium carbonate crystals. The VWA domain is conserved in the SMPs of various mollusk species; however, their phylogenetic relationship remains obscure. Furthermore, although the VWA domain participates in protein-protein interactions, its role in shell formation has not been established. Accordingly, in the current study, we investigate the phylogenetic relationship between PfuPif and other VWA domain-containing proteins in major mollusk species. The shell-related proteins containing VWA domains formed a large clade (the Pif/BMSP family) and were classified into eight subfamilies with unique sequential features, expression patterns, and taxa diversity. Furthermore, a pull-down assay using recombinant proteins containing the VWA domain of PfuPif 97 revealed that the VWA domain interacts with five nacreous layer-related SMPs of P. fucata, including Pif 80 and nacrein. Collectively, these results suggest that the VWA domain is important in the formation of organic complexes and participates in shell mineralisation.

摘要

Pif 是一种在珍珠贝（Pinctada fucata）珍珠层中发现的壳基质蛋白（SMP），由两种蛋白质组成，Pif97 和 Pif80。Pif97 含有一个 von Willebrand 因子 A（VWA）和几丁质结合结构域，而 Pif80 可以结合碳酸钙晶体。VWA 结构域在各种软体动物 SMP 中保守；然而，它们的系统发育关系仍然不清楚。此外，尽管 VWA 结构域参与蛋白-蛋白相互作用，但它在贝壳形成中的作用尚未确定。因此，在本研究中，我们研究了 PfuPif 和其他含有 VWA 结构域的软体动物主要物种 SMP 之间的系统发育关系。含有 VWA 结构域的壳相关蛋白形成了一个大的分支（Pif/BMSP 家族），并分为八个亚家族，具有独特的序列特征、表达模式和分类多样性。此外，使用含有 PfuPif 97 的 VWA 结构域的重组蛋白进行的下拉实验表明，VWA 结构域与珍珠贝的五种珍珠层相关 SMP 相互作用，包括 Pif80 和 nacrein。总之，这些结果表明 VWA 结构域在有机复合物的形成中很重要，并参与了贝壳矿化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/0f8d58436320/239_2024_10180_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/d3ebcd1d388a/239_2024_10180_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/62449bd3954d/239_2024_10180_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/fb901fdcb457/239_2024_10180_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/8b0f4a9cd852/239_2024_10180_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/0f8d58436320/239_2024_10180_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/d3ebcd1d388a/239_2024_10180_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/62449bd3954d/239_2024_10180_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/fb901fdcb457/239_2024_10180_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/8b0f4a9cd852/239_2024_10180_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/355e/11291548/0f8d58436320/239_2024_10180_Fig5_HTML.jpg

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