[HCFU和TNP 470对BALB/c腹膜后肉瘤肝转移（LMFS）的影响]

[Effects of HCFU and TNP 470 on liver metastasis of BALB/c retroperitoneal sarcoma (LMFS)].

作者信息

Fujii Y, Iwasa H, Hirai J, Hasumi K, Eriguchi M

机构信息

Dept. of Surgery, University of Tokyo.

出版信息

Gan To Kagaku Ryoho. 1999 Nov;26(13):2061-7.

PMID:10584572

Abstract

Combination therapy using HCFU and TNP 470, which inhibits angiogenesis, was examined for effectiveness against the footpad injection model of LMFS tumor. This LMFS, a retroperitoneal sarcoma of BALB/c mice, proliferated at the inoculation site (100% take) and all mice operated on after day 15 had spontaneous metastatic nodules in the liver. Mice with the LMFS tumor were given HCFU and 5-FU (5 days/week), and/or TNP 470 (3 days/week) from day 3 for 3 weeks and sacrified at day 28 under anesthesia. Seven of 10 mice receiving 5-FU and TNP 470 died from the side effects of the drugs. Mean tumor weight and liver metastatic nodules were determined and compared with a control group. The results were as follows: HCFU group: 94.6%, 11.8%, 5 FU group: 73.9%, 28.8%, TNP 470 group: 67.6%, 44%, HCFU and TNP 470 group: 33.3%, 6.4%. Mice with LMFS were given HCFU and/or TNP 470 from day 3 for 4 weeks. The foot on the injected side was amputated on day 15, and the animals were sacrified on day 35. Liver metastatic nodules compared with those of the operation (OP) group as follows: OP + HCFU group: 14.4%, OP + TNP group: 39.1%, and OP + HCFU + TNP 470 group: 5.4%. Histologically, 5 of 5 mice of the OP group, 3 of 5 of the OP + HCFU group, 4 of 5 of the OP + TNP 470 group and 1 of 5 of the OP + HCFU + TNP 470 group had liver metastases. These results show that while either HCFU or TNP 470 is effective by itself, a combination of these drugs is more effective against liver metastasis.

摘要

研究了使用HCFU和抑制血管生成的TNP 470进行联合治疗对LMFS肿瘤足垫注射模型的有效性。这种LMFS是BALB/c小鼠的腹膜后肉瘤，在接种部位增殖（接种成功率100%），并且在第15天之后接受手术的所有小鼠在肝脏中都有自发转移结节。患有LMFS肿瘤的小鼠从第3天开始接受HCFU和5-氟尿嘧啶（5天/周），和/或TNP 470（3天/周），持续3周，并在第28天麻醉下处死。接受5-氟尿嘧啶和TNP 470的10只小鼠中有7只因药物副作用死亡。测定平均肿瘤重量和肝脏转移结节，并与对照组进行比较。结果如下：HCFU组：94.6%，11.8%；5-氟尿嘧啶组：73.9%，28.8%；TNP 470组：67.6%，44%；HCFU和TNP 470组：33.3%，6.4%。患有LMFS的小鼠从第3天开始接受HCFU和/或TNP 470，持续4周。在第15天切除注射侧的足，动物在第35天处死。与手术（OP）组相比，肝脏转移结节情况如下：OP + HCFU组：14.4%，OP + TNP组：39.1%，OP + HCFU + TNP 470组：5.4%。组织学检查显示，OP组的5只小鼠中有5只、OP + HCFU组的5只中有3只、OP + TNP 470组的5只中有4只、OP + HCFU + TNP 470组的5只中有1只发生肝脏转移。这些结果表明，虽然HCFU或TNP 470单独使用时有效，但这两种药物联合使用对肝脏转移更有效。