Lanfredi-Rangel A, Kattenbach W M, Diniz J A, de Souza W
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28015-620, Campos dos Goytacazes, Rio de Janeiro, Brazil.
FEMS Microbiol Lett. 1999 Dec 15;181(2):245-51. doi: 10.1111/j.1574-6968.1999.tb08851.x.
Trophozoites of the primitive protozoan Giardia lamblia have been considered as cells which do not present the Golgi complex. Using C(6)-NBD ceramide, which has been shown to label the Golgi complex of mammalian cells, labelling of the perinuclear region of G. lamblia was observed by confocal laser scanning microscopy. Transmission electron microscopy of thin sections and of replicas of freeze-fractured cells revealed the presence of concentric perinuclear membranes resembling the Golgi complex.
原始原生动物蓝氏贾第鞭毛虫的滋养体曾被认为是不存在高尔基体的细胞。使用已被证明可标记哺乳动物细胞高尔基体的C(6)-NBD神经酰胺,通过共聚焦激光扫描显微镜观察到蓝氏贾第鞭毛虫核周区域有标记。对超薄切片和冷冻断裂细胞复制品的透射电子显微镜观察显示存在类似于高尔基体的同心核周膜。