Freni S C
Acta Cytol. 1975 Sep-Oct;19(5):448-52.
An experiment was carried out with the fluorescence impulse cytophotometer I.C.P. 11 (Phywe, Göttingen, Germany) in a routine cytology laboratory. A total of 600 cervical smears were examined with 15 test samples of body fluids and tissue cultures. The results were compared with those obtained by classical Papanicolaou cytology and clinical "follow-up". The main purpose was to find criteria for malignancy to be used as parameters in automated non-visual cytology. The main problems in the technique of preparing and measuring specimens have been discussed. Preparation of cell suspensions was time consuming; moreover, techniques were not completely controllable. A method for judging histograms was discussed. It appears that unless a better method is found for preparing suspensions of single, naked and undamaged nuclei, free from leukocytes, impulse cytophotometric measurement of DNA will remain an unreliable method for prescreening of cervical smears.
在一个常规细胞学实验室中，使用I.C.P. 11型荧光脉冲细胞光度计（德国哥廷根的Phywe公司生产）进行了一项实验。总共对600份宫颈涂片以及15份体液和组织培养的测试样本进行了检查。将结果与通过经典巴氏细胞学检查和临床“随访”获得的结果进行了比较。主要目的是寻找恶性肿瘤的标准，以便在自动化非视觉细胞学中用作参数。讨论了制备和测量标本技术中的主要问题。细胞悬液的制备耗时；此外，技术也不完全可控。讨论了一种判断直方图的方法。看来，除非找到一种更好的方法来制备不含白细胞的单个、裸露且未受损细胞核的悬液，否则DNA的脉冲细胞光度测量将仍然是一种不可靠的宫颈涂片预筛查方法。
