胎牛胸腺表达的V(D)J重组信号序列DNA结合活性。

V(D)J recombinational signal sequence DNA binding activities expressed by fetal bovine thymus.

作者信息

Zhu L, Halligan B D

机构信息

Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee 53226, USA.

出版信息

Vet Immunol Immunopathol. 1999 Nov 30;71(3-4):277-89. doi: 10.1016/s0165-2427(99)00099-9.

DOI:10.1016/s0165-2427(99)00099-9

PMID:10587307

Abstract

V(D)J recombination, or immunoglobulin gene rearrangement is a developmentally regulated, cell type specific, site directed recombination event that brings either immunoglobulin or T-cell receptor gene segments together to form mature, expressible Ig or TCT genes. This DNA recombination is directed by the recombinational signal sequences or RSS elements present adjacent to Ig and TCR gene segments. The RSS element is composed of a conserved nonamer element and a conserved heptamer element separated by a conserved length spacer region. In this report, we examine the expression of DNA binding proteins that interact with the RSS element in the bovine fetal thymus using EMSA assays. Our data indicates that the nonamer portion of the RSS element is the primary site of recognition for RSS binding proteins expressed in the bovine fetal thymus. We also show that these proteins are expressed from early stages of bovine fetal development through to full term development.

摘要

V(D)J重组，即免疫球蛋白基因重排，是一种受发育调控、细胞类型特异性、位点定向的重组事件，它将免疫球蛋白或T细胞受体基因片段聚集在一起，形成成熟的、可表达的Ig或TCR基因。这种DNA重组由位于Ig和TCR基因片段相邻位置的重组信号序列或RSS元件引导。RSS元件由一个保守的九聚体元件和一个保守的七聚体元件组成，二者被一个保守长度的间隔区隔开。在本报告中，我们使用电泳迁移率变动分析（EMSA）试验检测了牛胎儿胸腺中与RSS元件相互作用的DNA结合蛋白的表达。我们的数据表明，RSS元件的九聚体部分是牛胎儿胸腺中表达的RSS结合蛋白的主要识别位点。我们还表明，这些蛋白从牛胎儿发育的早期阶段一直到足月发育阶段都有表达。