细胞类型特异性染色质结构在体外决定V(D)J重组酶活性的靶向作用。

Cell type-specific chromatin structure determines the targeting of V(D)J recombinase activity in vitro.

作者信息

Stanhope-Baker P, Hudson K M, Shaffer A L, Constantinescu A, Schlissel M S

机构信息

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

出版信息

Cell. 1996 Jun 14;85(6):887-97. doi: 10.1016/s0092-8674(00)81272-6.

DOI:10.1016/s0092-8674(00)81272-6

PMID:8681383

Abstract

A common V(D)J recombinase that recognizes a conserved recombination signal sequence (RSS) mediates the assembly of immunoglobulin (Ig) and T cell receptor (TCR) genes in B and T cell precursors. The rearrangement of particular Ig and TCR gene segments, however, is tightly regulated with respect to cell lineage and developmental stage. Using an in vitro system, we analyzed recombinase cleavage of RSSs flanking Ig and TCR gene segments in nuclei. We found that both the lineage-specificity and temporal ordering of gene rearrangement is reflected in the accessibility of RSSs within chromatin to in vitro cleavage.

摘要

一种识别保守重组信号序列（RSS）的常见V（D）J重组酶介导B细胞和T细胞前体中免疫球蛋白（Ig）和T细胞受体（TCR）基因的组装。然而，特定Ig和TCR基因片段的重排相对于细胞谱系和发育阶段受到严格调控。我们使用体外系统分析了细胞核中Ig和TCR基因片段侧翼RSS的重组酶切割情况。我们发现染色质内RSS对体外切割的可及性反映了基因重排的谱系特异性和时间顺序。