Kamoun S, Hraber P, Sobral B, Nuss D, Govers F
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA.
Fungal Genet Biol. 1999 Nov;28(2):94-106. doi: 10.1006/fgbi.1999.1166.
A total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes.
利用从致病疫霉菌丝体RNA构建的cDNA文库中的克隆进行随机测序，共鉴定出1000个对应于760个独特序列集的表达序列标签（EST）。开发了许多以关系数据库和分析流程为代表的软件程序，用于EST序列数据的自动化分析和存储。一组419个非冗余序列（共对应632个EST，占63.2%）被鉴定为与公共数据库中存储的序列有显著匹配。为所有419个序列指定了假定的细胞身份和功能。所有主要功能类别都至少有几个EST代表。四个新的cDNA含有与激发素相关的序列，激发素是一类结构相关的蛋白质家族，可诱导过敏反应并决定致病疫霉在烟草植物上的无毒力，它们是鉴定出的最显著的基因之一。其中两个类激发素cDNA是检测到的最丰富的cDNA之一。该序列集还包含几个与独特植物基因具有高度序列相似性的EST。
