Schneider K, Borchardt D C, Schäfer-Pregl R, Nagl N, Glass C, Jeppsson A, Gebhardt C, Salamini F
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Mol Gen Genet. 1999 Oct;262(3):515-24. doi: 10.1007/s004380051113.
To analyse genetic factors that potentially affect sugar quality and yield in Beta vulgaris, we designed primers based on 18 homologous ESTs and conserved regions of 32 heterologous ESTs encoding gene products that act in the Calvin cycle, the oxidative pentose phosphate cycle, photorespiration, synthesis, transport and degradation of sucrose, glycolysis, the citric acid cycle, nitrogen metabolism and osmoprotection. Data on the amplification of 54 gene homologues from B. vulgaris are presented. Among these are 35 homologues for which DNA sequence information from B. vulgaris is now available for the first time. For genetic mapping a PCR-based strategy using CAPS (cleaved amplified polymorphic sequence), DFLP (DNA fragment length polymorphism), SSCP (single-strand conformation polymorphism) and HD (heteroduplex) analysis was adopted. RFLP analysis was also used in some cases. The different techniques used for the detection of polymorphisms are evaluated with respect to their sensitivity and versatility. In all, 42 functional genes have been assigned to the nine linkage groups of sugar beet.
为分析可能影响甜菜糖品质和产量的遗传因素，我们基于18个同源EST以及32个异源EST的保守区域设计了引物，这些EST编码参与卡尔文循环、氧化戊糖磷酸循环、光呼吸、蔗糖合成、运输和降解、糖酵解、柠檬酸循环、氮代谢和渗透保护的基因产物。本文给出了从甜菜中扩增54个基因同源物的数据。其中35个同源物的甜菜DNA序列信息首次可得。对于基因定位，采用了基于PCR的策略，包括CAPS（酶切扩增多态性序列）、DFLP（DNA片段长度多态性）、SSCP（单链构象多态性）和HD（异源双链）分析。在某些情况下也使用了RFLP分析。对用于检测多态性的不同技术的灵敏度和通用性进行了评估。总共42个功能基因已被定位到甜菜的9个连锁群上。
