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将腺相关病毒掺入磷酸钙共沉淀物中可改善体外和体内基因向气道上皮细胞的转移。

Incorporation of adeno-associated virus in a calcium phosphate coprecipitate improves gene transfer to airway epithelia in vitro and in vivo.

作者信息

Walters R W, Duan D, Engelhardt J F, Welsh M J

机构信息

Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.

出版信息

J Virol. 2000 Jan;74(1):535-40. doi: 10.1128/jvi.74.1.535-540.2000.

DOI:10.1128/jvi.74.1.535-540.2000
PMID:10590145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC111567/
Abstract

Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaP(i) coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.

摘要

腺相关病毒（AAV）由于顶端表面缺乏受体，在感染分化的气道上皮细胞时效率低下。我们推测，将AAV掺入磷酸钙共沉淀物中可以克服这一障碍。有趣的是，2型AAV的共沉淀提高了体外向分化的人气道上皮细胞以及体内向小鼠肺的基因转移效率。这些结果表明，以磷酸钙共沉淀物形式递送AAV可能会显著提高其在体内向气道上皮细胞进行基因转移的效用。

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Incorporation of adeno-associated virus in a calcium phosphate coprecipitate improves gene transfer to airway epithelia in vitro and in vivo.将腺相关病毒掺入磷酸钙共沉淀物中可改善体外和体内基因向气道上皮细胞的转移。
J Virol. 2000 Jan;74(1):535-40. doi: 10.1128/jvi.74.1.535-540.2000.
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本文引用的文献

1
Mechanism by which calcium phosphate coprecipitation enhances adenovirus-mediated gene transfer.磷酸钙共沉淀增强腺病毒介导的基因转移的机制。
Gene Ther. 1999 Nov;6(11):1845-50. doi: 10.1038/sj.gt.3301020.
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Enhancement of calcium phosphate-mediated transfection by inclusion of adenovirus in coprecipitates.通过在共沉淀物中加入腺病毒增强磷酸钙介导的转染。
Gene Ther. 1999 Apr;6(4):676-82. doi: 10.1038/sj.gt.3300857.
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Adeno-associated virus 2 co-receptors?腺相关病毒2型共受体？
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Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia.纤维受体的基底外侧定位限制了腺病毒从气道上皮细胞顶端表面的感染。
J Biol Chem. 1999 Apr 9;274(15):10219-26. doi: 10.1074/jbc.274.15.10219.
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Hum Gene Ther. 1999 Mar 1;10(4):603-13. doi: 10.1089/10430349950018670.
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Human fibroblast growth factor receptor 1 is a co-receptor for infection by adeno-associated virus 2.人类成纤维细胞生长因子受体1是2型腺相关病毒感染的共受体。
Nat Med. 1999 Jan;5(1):71-7. doi: 10.1038/4758.
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Cloning and characterization of adeno-associated virus type 5.5型腺相关病毒的克隆与特性分析
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Polarity influences the efficiency of recombinant adenoassociated virus infection in differentiated airway epithelia.极性影响重组腺相关病毒在分化气道上皮细胞中的感染效率。
Hum Gene Ther. 1998 Dec 10;9(18):2761-76. doi: 10.1089/hum.1998.9.18-2761.
10
Successful readministration of adeno-associated virus vectors to the mouse lung requires transient immunosuppression during the initial exposure.将腺相关病毒载体成功重新给药至小鼠肺部需要在初次暴露期间进行短暂的免疫抑制。
J Virol. 1998 Dec;72(12):9795-805. doi: 10.1128/JVI.72.12.9795-9805.1998.