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酿酒酵母中一些PHO基因的转录受spt7p调控。

Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by spt7p.

作者信息

Nishimura K, Yasumura K, Igarashi K, Harashima S, Kakinuma Y

机构信息

Faculty of Pharmaceutical Sciences, Chiba University, Japan.

出版信息

Yeast. 1999 Dec;15(16):1711-7. doi: 10.1002/(SICI)1097-0061(199912)15:16<1711::AID-YEA497>3.0.CO;2-8.

Abstract

Spt7p is a new global transcription factor in Saccharomyces cerevisiae(Gansheroff et al., 1995). We report here that the activities of high affinity phosphate transport and acid phosphatase in particular were decreased in a spt7 null mutant. Northern blot experiments revealed that transcription of the PHO84 and PHO5 genes was impaired in this mutant; expression of the PHO regulatory genes, PHO4 and PHO2, was normal. Spt7p is thus linked with expression of several structural genes of the PHO regulon in yeast.

摘要

Spt7p是酿酒酵母中的一种新型全局转录因子(甘舍罗夫等人,1995年)。我们在此报告,在spt7基因缺失突变体中,尤其是高亲和力磷酸盐转运和酸性磷酸酶的活性降低。Northern印迹实验表明,该突变体中PHO84和PHO5基因的转录受到损害;PHO调控基因PHO4和PHO2的表达正常。因此,Spt7p与酵母中PHO调节子的几个结构基因的表达相关。

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