Becvar J E, Hastings J W
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3374-6. doi: 10.1073/pnas.72.9.3374.
Recent reports revive a hypothesis that the bacterial bioluminescence reaction involves two reduced flavin mononucleotide molecules per enzyme turnover. A two-flavin mechanism requires that the two flavins bind simultaneously or sequentially to the same or different sites on luciferase during a catalytic cycle. Measurements using equilibrium techniques show that the luciferase dimer has only a single reduced flavin binding site. Quantum yield results demonstrate that bioluminescence requires only one reduced flavin per luciferase, ruling out mechanisms involving either two reduced flavins or one reduced flavin plus one oxidized flavin per catalytic cycle.
近期的报告重新提出了一种假说，即细菌生物发光反应在每次酶促反应周转过程中涉及两个还原型黄素单核苷酸分子。双黄素机制要求这两个黄素在催化循环过程中同时或依次结合到荧光素酶上相同或不同的位点。使用平衡技术进行的测量表明，荧光素酶二聚体只有一个还原型黄素结合位点。量子产率结果表明，生物发光每个荧光素酶只需要一个还原型黄素，排除了每个催化循环涉及两个还原型黄素或一个还原型黄素加一个氧化型黄素的机制。
