被λ原噬菌体抑制的T7噬菌体突变体。
Mutants of T7 bacteriophage inhibited by lambda prophage.
作者信息
Pao C C, Speyer J F
出版信息
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3642-6. doi: 10.1073/pnas.72.9.3642.
Abstract
Mutants in gene 20, a new T7 gene, cannot grow on rex+ lambda lysogens. Gene 20-- mutants suppress in double mutants the phenotype of T7 ligase negative mutations, but not vice versa. Amber 20- mutants have been obtained. There are differences between these T7 mutations and the similar T4 rII mutations. There are host mutations which permit T7 20- mutants to grow on lambda+ lysogens. T7 DNA synthesis on normal lambda+ lysogens infected with 20- mutants is essentially normal, but the DNA is not packaged. The gene 20 protein is active in in vitro complementation and probably used late in infection for DNA packaging into phage heads.
摘要
基因20（一个新的T7基因）的突变体无法在rex⁺ λ溶原菌上生长。基因20⁻突变体在双突变体中可抑制T7连接酶阴性突变的表型，但反之则不然。已获得琥珀型20⁻突变体。这些T7突变与类似的T4 rII突变之间存在差异。存在一些宿主突变，可使T7 20⁻突变体在λ⁺溶原菌上生长。用20⁻突变体感染正常的λ⁺溶原菌后，T7 DNA合成基本正常，但DNA未被包装。基因20蛋白在体外互补中具有活性，可能在感染后期用于将DNA包装到噬菌体头部。
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