利用玉米基因组数据库ZmDB进行基因发现。
Gene discovery using the maize genome database ZmDB.
作者信息
Gai X, Lal S, Xing L, Brendel V, Walbot V
机构信息
Department of Zoology, Iowa State University, Ames, IA 50011-3260, USA.
出版信息
Nucleic Acids Res. 2000 Jan 1;28(1):94-6. doi: 10.1093/nar/28.1.94.
Abstract
Zea mays DataBase (ZmDB) is a repository and analysis tool for sequence, expression and phenotype data of the major crop plant maize. The data accessible in ZmDB are mostly generated in a large collaborative project of maize gene discovery, sequencing and phenotypic analysis using a transposon tagging strategy and expressed sequence tag (EST) sequencing. ESTs constitute most of the current content. Database search tools, convenient links to external databases, and novel sequence analysis programs for spliced alignment are provided and together serve as an efficient protocol for gene discovery by sequence inspection. ZmDB can be accessed at http://zmdb. iastate.edu. ZmDB also provides web-based ordering of materials generated in the project, including EST and genomic DNA clones, seeds of mutant plants and microarrays of amplified EST and genomic DNA sequences.
摘要
玉米数据库（ZmDB）是一个用于存储和分析主要农作物玉米的序列、表达和表型数据的资源库及分析工具。ZmDB中可获取的数据大多产生于一个大型玉米基因发现、测序和表型分析合作项目，该项目采用转座子标签策略和表达序列标签（EST）测序技术。EST构成了当前数据库的大部分内容。数据库提供了搜索工具、与外部数据库的便捷链接以及用于剪接比对的新型序列分析程序，这些共同构成了通过序列检查进行基因发现的有效方案。可通过http://zmdb.iastate.edu访问ZmDB。ZmDB还提供基于网络的项目所产生材料的订购服务，包括EST和基因组DNA克隆、突变体植物种子以及扩增的EST和基因组DNA序列微阵列。
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