Park S C, Choi Y W, Oh T K
Microbial Enzyme RU, Korea Research Institute of Bioscience & Biotechnology, Yusong, Taejon.
J Vet Med Sci. 1999 Nov;61(11):1257-9. doi: 10.1292/jvms.61.1257.
Bacillus amyloliquefaciens DS11 phytase (DS11 phytase) and Aspergillus ficuum phytase (AF phytase) activities were investigated by measuring the release of phosphate from phytate in animal feedstuff such as wheat bran, corn meal, soybean meal and rice flour at pH 5 and 7. In all the tested feedstuff, the enzymatic activity of DS11 phytase was more active at pH 7, but that of AF phytase was more active at pH 5. From these results, the phytate in the gastrointestinal tract could be degraded in the small intestine or stomach by DS11 or AF phytase, respectively. In conclusion, the results presented in this paper indicated that different combination ratios of DS11 and AF phytase, depending on the kind of feedstuff, might effectively induce more enzymatic activity both in the stomach and small intestine in terms of the pH of the gastrointestinal tract.
通过在pH值为5和7的条件下，测量植酸酶从动物饲料（如麦麸、玉米粉、豆粕和米粉）中的植酸盐释放磷酸盐的量，对解淀粉芽孢杆菌DS11植酸酶（DS11植酸酶）和泡盛曲霉植酸酶（AF植酸酶）的活性进行了研究。在所有测试的饲料中，DS11植酸酶在pH 7时酶活性更高，而AF植酸酶在pH 5时酶活性更高。根据这些结果，胃肠道中的植酸盐可分别被DS11或AF植酸酶在小肠或胃中降解。总之，本文给出的结果表明，根据饲料种类不同，DS11和AF植酸酶的不同组合比例可能会根据胃肠道的pH值，在胃和小肠中有效诱导出更高的酶活性。
