Scranton P E, McMaster J H, Diamond P E
Clin Orthop Relat Res. 1975 Oct(112):340-8.
Recent evidence suggests that endocrine factors play an important role in the natural history of osteosarcoma. The occurrence of this tumor in the metaphysis of rapidly growing adolescents, coupled with increased female survival led to the investigation of the effects of various hormones on cultured osteosarcoma cells. The in vitro effects of physiologic concentrations of human growth hormone, 17beta estradiol, and progesterone on cultured osteosarcoma cells and chondrocytes are presented. Growth hormone significantly enhances 3H-thymidine incorporation in osteosarcoma cells and chondrocytes, in the presence of human serum. The use of other sera, culture media, or heat inactivation of the human serum abolishes this effect. Estradiol and progesterone, alone, or in combination produce significant suppression of DNA synthesis in cultured tumor cells. Several sera contain a heat-labile factor which has the capacity to block the suppressive effect of estradiol. This factor could be overcome by increasing the concentration of hormone, or by heat-inactivation of the serum. The use of hormone therapy in the treatment of osteosarcoma has never been reported, despite its demonstrated value in certain other malignancies. In light of these observations and considering the poor prognosis in this disease it seems reasonable to initiate a study of adjunctive hormone therapy in osteosarcama.
最近有证据表明，内分泌因素在骨肉瘤的自然病程中起重要作用。这种肿瘤发生在快速生长的青少年的干骺端，加上女性生存率的提高，促使人们研究各种激素对培养的骨肉瘤细胞的影响。本文介绍了生理浓度的人生长激素、17β-雌二醇和孕酮对培养的骨肉瘤细胞和软骨细胞的体外作用。在人血清存在的情况下，生长激素显著增强骨肉瘤细胞和软骨细胞对3H-胸腺嘧啶核苷的摄取。使用其他血清、培养基或对人血清进行热灭活会消除这种作用。单独或联合使用雌二醇和孕酮可显著抑制培养的肿瘤细胞中的DNA合成。几种血清含有一种热不稳定因子，它能够阻断雌二醇的抑制作用。可以通过增加激素浓度或对血清进行热灭活来克服这种因子。尽管激素疗法在某些其他恶性肿瘤中已显示出价值，但从未有过关于其用于治疗骨肉瘤的报道。鉴于这些观察结果，并考虑到这种疾病的预后较差，启动一项关于骨肉瘤辅助激素治疗的研究似乎是合理的。
