Frazier R A, Ames J M, Nursten H E
Department of Food Science and Technology, The University of Reading, Whiteknights, UK.
Electrophoresis. 1999 Oct;20(15-16):3156-80. doi: 10.1002/(SICI)1522-2683(19991001)20:15/16<3156::AID-ELPS3156>3.0.CO;2-T.
Capillary electrophoresis (CE) offers the analyst a number of key advantages for the analysis of the components of foods. CE offers better resolution than, say, high-performance liquid chromatography (HPLC), and is more adept at the simultaneous separation of a number of components of different chemistries within a single matrix. In addition, CE requires less rigorous sample cleanup procedures than HPLC, while offering the same degree of automation. However, despite these advantages, CE remains under-utilized by food analysts. Therefore, this review consolidates and discusses the currently reported applications of CE that are relevant to the analysis of foods. Some discussion is also devoted to the development of these reported methods and to the advantages/disadvantages compared with the more usual methods for each particular analysis. It is the aim of this review to give practicing food analysts an overview of the current scope of CE.
毛细管电泳(CE)为分析人员提供了许多用于食品成分分析的关键优势。与例如高效液相色谱法(HPLC)相比,CE具有更高的分辨率,并且更擅长在单一基质中同时分离多种不同化学性质的成分。此外,与HPLC相比,CE所需的样品净化程序不那么严格,同时具有相同程度的自动化。然而,尽管有这些优势,CE在食品分析人员中的应用仍然不足。因此,本综述汇总并讨论了目前报道的与食品分析相关的CE应用。还对这些报道方法的发展以及与每种特定分析的更常用方法相比的优缺点进行了一些讨论。本综述的目的是让从事食品分析的人员对CE的当前应用范围有一个概述。