Chiba K, Sone T, Kawakami K, Onoue M
Yakult Institute for Microbiological Research, Tokyo, Japan.
Exp Dermatol. 1999 Dec;8(6):471-9. doi: 10.1111/j.1600-0625.1999.tb00305.x.
The present study examines the cumulative effects of sub-erythema application of squalene-monohydroperoxide (Sq-OOH), the initial products of UV-peroxidated squalene, to the skin of hairless mice. Sq-OOH was isolated by the methanol extraction and preparative HPLC method. Repeated topical application of 10 mM Sq-OOH to hairless mice for 3 weeks induced definite skin roughness and crinkle formation. 3-D surface parameter analysis revealed changes in all roughness parameters (number of furrows and crests, distance between a furrow and next crest, and irregularity) of the group treated with more than 3 mM Sq-OOH compared to the control group. These skin surface changes were not induced by squalene, squalene-monohydroxide (Sq-OH) or organic hydroperoxides such as tert-butyl hydroperoxide and cumene-hydroperoxide at 10 mM. Similarly, such changes were not induced by primary irritants, such as sodium lauryl sulfate and n-tetradecane under the same experimental conditions. Skin conductance decreased, following application of 10 mM Sq-OOH. Histological observation revealed that application of 10 mM Sq-OOH induced slight hyperkeratosis, moderate epidermal thickening and slight hyperplasia of sebaceous glands.
本研究考察了角鲨烯单氢过氧化物（Sq - OOH），即紫外线过氧化角鲨烯的初始产物，对无毛小鼠皮肤进行亚红斑剂量涂抹的累积效应。通过甲醇萃取和制备型高效液相色谱法分离出Sq - OOH。对无毛小鼠连续3周局部涂抹10 mM Sq - OOH会导致明显的皮肤粗糙和皱纹形成。三维表面参数分析显示，与对照组相比，用超过3 mM Sq - OOH处理的组的所有粗糙度参数（皱纹和嵴的数量、一条皱纹与下一条嵴之间的距离以及不规则度）均发生了变化。角鲨烯、角鲨烯单氢氧化物（Sq - OH）或有机过氧化物如叔丁基过氧化氢和异丙苯过氧化氢以10 mM的浓度处理时不会引起这些皮肤表面变化。同样，在相同实验条件下，主要刺激物如十二烷基硫酸钠和正十四烷也不会引起这种变化。涂抹10 mM Sq - OOH后皮肤导电性降低。组织学观察显示，涂抹10 mM Sq - OOH会导致轻微的角化过度、中度的表皮增厚以及皮脂腺的轻微增生。
