Cerná Z, Kolárová I
Folia Parasitol (Praha). 1978;25(4):289-92.
The importance of the IFA-test (Indirect Fluorescent Antibody Test) in the diagnosis of sarcocystosis in the intermediate host has been confirmed in the present paper. We assessed the time of the first appearance of serum antibodies in the intermediate host in mice inoculated experimentally with the species Sarcocystis dispersa. By means of the IFA-test, the first antibodies were found on day 20 p.i. Cross-reactions among antisera of S. dispersa and a heterologous antigen of S. cernae disclosed that the reaction was not species-specific, but genus-specific. In addition, we confirmed serologically that the antigenic structure of the genus Frenkelia was identical to that of the genus Sarcocystis, because the results of cross reactions obtained with the IFA-test were identical.
本文证实了间接荧光抗体试验(IFA试验)在中间宿主肉孢子虫病诊断中的重要性。我们评估了用分散肉孢子虫实验接种的小鼠中间宿主中血清抗体首次出现的时间。通过IFA试验,在感染后第20天发现了首批抗体。分散肉孢子虫抗血清与猪囊尾蚴异源抗原之间的交叉反应表明,该反应不是种特异性的,而是属特异性的。此外,我们通过血清学证实,弗氏属的抗原结构与肉孢子虫属相同,因为IFA试验获得的交叉反应结果是相同的。