使用简并引物通过聚合酶链反应检测水痘带状疱疹病毒和单纯疱疹病毒。

Detection of varicella-zoster virus and herpes simplex virus by the polymerase chain reaction with degenerate primers.

作者信息

Jacobs J J, Folkers E, Vreeswijk J

机构信息

Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.

出版信息

J Virol Methods. 1999 Dec;83(1-2):155-67. doi: 10.1016/s0166-0934(99)00118-4.

DOI:10.1016/s0166-0934(99)00118-4

PMID:10598093

Abstract

Varicella-zoster virus (VZV) and herpes simplex virus (HSV) are human pathogens of significance involved in multiple diseases with either typical or atypical clinical features. In neonates and immunocompromised patients these alphaherpesviruses may cause life-threatening diseases such as encephalitis. Detection of VZV by virus culture is difficult. Polymerase chain reaction (PCR) is quicker and more sensitive and applicable in most clinical microbiological laboratories. Using degenerate primers, glycoprotein B (gB) DNA was amplified from all alphaherpesvirus field strains present in clinical samples. The amplification of gB allowed virus typing of VZV, HSV-1 and HSV-2 using restriction enzyme digestion of the PCR products. Degenerate primers can replace conventional primers in diagnostic PCR without loss of sensitivity and specificity.

摘要

水痘带状疱疹病毒（VZV）和单纯疱疹病毒（HSV）是重要的人类病原体，可引发多种具有典型或非典型临床特征的疾病。在新生儿和免疫功能低下的患者中，这些α疱疹病毒可能会导致危及生命的疾病，如脑炎。通过病毒培养检测VZV很困难。聚合酶链反应（PCR）更快、更灵敏，并且适用于大多数临床微生物实验室。使用简并引物，从临床样本中存在的所有α疱疹病毒野外菌株中扩增出糖蛋白B（gB）DNA。gB的扩增使得通过对PCR产物进行限制性酶切消化来对VZV、HSV-1和HSV-2进行病毒分型成为可能。简并引物可以在诊断性PCR中替代传统引物，而不会损失敏感性和特异性。