Bella J, Rossmann M G
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
J Struct Biol. 1999 Dec 1;128(1):69-74. doi: 10.1006/jsbi.1999.4143.
The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen or macrophage-1 antigen. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry. The three-dimensional atomic structure of the two amino-terminal domains (D1 and D2) of ICAM-1 has been determined to 2.2 A resolution and fitted into a cryoelectron microscopy reconstruction of a rhinovirus-ICAM-1 complex. Rhinovirus attachment is confined to the BC, CD, DE, and FG loops of the amino-terminal Ig-like domain (D1) at the end distal to the cellular membrane. The loops are considerably different in structure to those of human ICAM-2 or murine ICAM-1, which do not bind rhinoviruses. There are extensive charge interactions between ICAM-1 and human rhinoviruses, which are mostly conserved in both major and minor receptor groups of rhinoviruses.
人细胞间黏附分子-1（ICAM-1）的正常功能是在损伤或应激后提供内皮细胞与白细胞之间的黏附作用。ICAM-1与白细胞功能相关抗原或巨噬细胞-1抗原结合。然而，ICAM-1也是主要的人类鼻病毒群的受体，并且是病毒进入细胞过程中随后病毒脱壳的催化剂。已确定ICAM-1的两个氨基末端结构域（D1和D2）的三维原子结构分辨率为2.2埃，并将其拟合到鼻病毒-ICAM-1复合物的冷冻电子显微镜重建模型中。鼻病毒的附着局限于细胞膜远端的氨基末端免疫球蛋白样结构域（D1）的BC、CD、DE和FG环。这些环的结构与不结合鼻病毒的人ICAM-2或小鼠ICAM-1的环有很大不同。ICAM-1与人类鼻病毒之间存在广泛的电荷相互作用，这在鼻病毒的主要和次要受体组中大多是保守的。
