嗜硫还原地杆菌的周质9.6千道尔顿c型细胞色素不是向Fe(III)传递电子的穿梭载体。
The periplasmic 9.6-kilodalton c-type cytochrome of Geobacter sulfurreducens is not an electron shuttle to Fe(III).
作者信息
Lloyd J R, Blunt-Harris E L, Lovley D R
机构信息
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
出版信息
J Bacteriol. 1999 Dec;181(24):7647-9. doi: 10.1128/JB.181.24.7647-7649.1999.
Abstract
Geobacter sulfurreducens contains a 9.6-kDa c-type cytochrome that was previously proposed to serve as an extracellular electron shuttle to insoluble Fe(III) oxides. However, when the cytochrome was added to washed-cell suspensions of G. sulfurreducens it did not enhance Fe(III) oxide reduction, whereas similar concentrations of the known electron shuttle, anthraquinone-2,6-disulfonate, greatly stimulated Fe(III) oxide reduction. Furthermore, analysis of the extracellular c-type cytochromes in cultures of G. sulfurreducens demonstrated that the dominant c-type cytochrome was not the 9.6-kDa cytochrome, but rather a 41-kDa cytochrome. These results and other considerations suggest that the 9.6-kDa cytochrome is not an important extracellular electron shuttle to Fe(III) oxides.
摘要
嗜硫还原地杆菌含有一种9.6千道尔顿的c型细胞色素，之前有人提出它可作为一种细胞外电子穿梭体作用于不溶性的Fe(III)氧化物。然而，当将这种细胞色素添加到嗜硫还原地杆菌的洗过的细胞悬液中时，它并未增强Fe(III)氧化物的还原作用，而类似浓度的已知电子穿梭体蒽醌-2,6-二磺酸盐却极大地刺激了Fe(III)氧化物的还原。此外，对嗜硫还原地杆菌培养物中的细胞外c型细胞色素的分析表明，占主导地位的c型细胞色素不是9.6千道尔顿的细胞色素，而是一种41千道尔顿的细胞色素。这些结果以及其他因素表明，9.6千道尔顿的细胞色素并非作用于Fe(III)氧化物的重要细胞外电子穿梭体。
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