Department of Microbiology, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Department of Mathematics and Statistics, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Microbiology (Reading). 2013 Mar;159(Pt 3):515-535. doi: 10.1099/mic.0.064089-0. Epub 2013 Jan 10.
Whole-genome microarray analysis of Geobacter sulfurreducens grown on insoluble Fe(III) oxide or Mn(IV) oxide versus soluble Fe(III) citrate revealed significantly different expression patterns. The most upregulated genes, omcS and omcT, encode cell-surface c-type cytochromes, OmcS being required for Fe(III) and Mn(IV) oxide reduction. Other electron transport genes upregulated on both metal oxides included genes encoding putative menaquinol : ferricytochrome c oxidoreductase complexes Cbc4 and Cbc5, periplasmic c-type cytochromes Dhc2 and PccF, outer membrane c-type cytochromes OmcC, OmcG and OmcV, multicopper oxidase OmpB, the structural components of electrically conductive pili, PilA-N and PilA-C, and enzymes that detoxify reactive oxygen/nitrogen species. Genes upregulated on Fe(III) oxide encode putative menaquinol : ferricytochrome c oxidoreductase complexes Cbc3 and Cbc6, periplasmic c-type cytochromes, including PccG and PccJ, and outer membrane c-type cytochromes, including OmcA, OmcE, OmcH, OmcL, OmcN, OmcO and OmcP. Electron transport genes upregulated on Mn(IV) oxide encode periplasmic c-type cytochromes PccR, PgcA, PpcA and PpcD, outer membrane c-type cytochromes OmaB/OmaC, OmcB and OmcZ, multicopper oxidase OmpC and menaquinone-reducing enzymes. Genetic studies indicated that MacA, OmcB, OmcF, OmcG, OmcH, OmcI, OmcJ, OmcM, OmcV and PccH, the putative Cbc5 complex subunit CbcC and the putative Cbc3 complex subunit CbcV are important for reduction of Fe(III) oxide but not essential for Mn(IV) oxide reduction. Gene expression patterns for Geobacter uraniireducens were similar. These results demonstrate that the physiology of Fe(III)-reducing bacteria differs significantly during growth on different insoluble and soluble electron acceptors and emphasize the importance of c-type cytochromes for extracellular electron transfer in G. sulfurreducens.
利用全基因组微阵列分析，研究了 Geobacter sulfurreducens 在不溶性 Fe(III)氧化物或 Mn(IV)氧化物与可溶性 Fe(III)柠檬酸盐上的生长情况，结果表明其表达模式存在显著差异。上调表达最显著的基因 omcS 和 omcT 编码细胞表面 c 型细胞色素，OmcS 是 Fe(III)和 Mn(IV)氧化物还原所必需的。在两种金属氧化物上上调表达的其他电子传输基因包括编码假定的menaquinol ：亚铁细胞色素 c 氧化还原酶复合物 Cbc4 和 Cbc5、周质 c 型细胞色素 Dhc2 和 PccF、外膜 c 型细胞色素 OmcC、OmcG 和 OmcV、多铜氧化酶 OmpB、电导率菌毛的结构成分 PilA-N 和 PilA-C 以及解毒活性氧/氮物种的酶。在 Fe(III)氧化物上上调表达的基因编码假定的menaquinol ：亚铁细胞色素 c 氧化还原酶复合物 Cbc3 和 Cbc6、周质 c 型细胞色素，包括 PccG 和 PccJ，以及外膜 c 型细胞色素，包括 OmcA、OmcE、OmcH、OmcL、OmcN、OmcO 和 OmcP。在 Mn(IV)氧化物上上调表达的电子传输基因编码周质 c 型细胞色素 PccR、PgcA、PpcA 和 PpcD、外膜 c 型细胞色素 OmaB/OmaC、OmcB 和 OmcZ、多铜氧化酶 OmpC 和menaquinone 还原酶。遗传研究表明，MacA、OmcB、OmcF、OmcG、OmcH、OmcI、OmcJ、OmcM、OmcV 和 PccH、假定的 Cbc5 复合物亚基 CbcC 和假定的 Cbc3 复合物亚基 CbcV 对 Fe(III)氧化物的还原很重要，但不是 Mn(IV)氧化物还原所必需的。Geobacter uraniireducens 的基因表达模式相似。这些结果表明，在不同的不溶性和可溶性电子受体上生长时，Fe(III)还原细菌的生理学有很大差异，并强调 c 型细胞色素对 G. sulfurreducens 细胞外电子转移的重要性。
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