乙肝病毒基因组：限制酶切割及从 Dane 颗粒中提取的 DNA 的结构
Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.
作者信息
Summers J, O'Connell A, Millman I
出版信息
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4597-601. doi: 10.1073/pnas.72.11.4597.
DNA extracted from Dane particles has been characterized by gel electrophoresis and restriction enzyme cleavage with endonuclease R-HaeIII (from Hemophilus aegyptius). Dane particle DNA is proposed to be a double-stranded circular DNA approximately 3600 nucleotides in length containing a single-stranded gap of 600-2100 nucleotides. The endogenous DNA polymerase (DNA nucleotidyl-transferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 2.7.7.7) reaction appears to repair this single-stranded gap.
从D型颗粒中提取的DNA已通过凝胶电泳和用R-HaeIII内切酶（来自埃及嗜血杆菌）进行限制性酶切分析其特征。D型颗粒DNA被认为是一种双链环状DNA，长度约为3600个核苷酸，含有一个600 - 2100个核苷酸的单链缺口。内源性DNA聚合酶（DNA核苷酸转移酶；脱氧核苷三磷酸：DNA脱氧核苷酸转移酶；EC 2.7.7.7）反应似乎可修复这个单链缺口。
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