Song J J, Kim J H, Lee H, Kim E, Kim J, Park Y S, Ahn J, Yoo N C, Roh J K, Kim B S
Institute for Cancer Research, Yonsei University College of Medicine, Seoul, Korea.
Oncol Rep. 2000 Jan-Feb;7(1):119-24. doi: 10.3892/or.7.1.119.
Retroviral vectors have recently experienced limited use in cancer gene therapy mainly due to poor transduction efficiency. To overcome this drawback, we attempted to enhance the transduction efficiency by employing different retroviral packaging cell lines and chemical additives. The retrovirus from the PG13 packaging cell line gave mostly higher or similar transduction efficiencies in a variety of human cancer cell lines compared to the retrovirus from the PA317, Bing, or FLYRD18 packaging cell line. A cationic liposome, especially Lipofectamine, significantly enhanced the transduction efficiency of a retrovirus. However, the retrovirus derived from the PG13 cell line could not infect the murine cell line efficiently even after Lipofectamine treatment. Furthermore, chloroquine did not improve the transduction efficiency regardless of the presence of chemical additives. These results, therefore, suggested that the transduction efficiency of a retrovirus in human cancer cells can certainly be improved when a proper packaging cell line is chosen. In addition, this study implied that Lipofectamine is a superb additive to enhance the transduction efficiency of a retrovirus via a specific virus envelope protein-receptor interaction for virus entry, and that receptor-mediated endocytosis does not seem to be the leading route of virus delivery to liberate a virus genome.
逆转录病毒载体近来在癌症基因治疗中的应用有限，主要原因是转导效率低下。为克服这一缺点，我们尝试通过使用不同的逆转录病毒包装细胞系和化学添加剂来提高转导效率。与来自PA317、Bing或FLYRD18包装细胞系的逆转录病毒相比，来自PG13包装细胞系的逆转录病毒在多种人类癌细胞系中的转导效率大多更高或相似。一种阳离子脂质体，尤其是Lipofectamine，能显著提高逆转录病毒的转导效率。然而，即使经过Lipofectamine处理，源自PG13细胞系的逆转录病毒仍无法有效感染小鼠细胞系。此外，无论是否存在化学添加剂，氯喹都不能提高转导效率。因此，这些结果表明，当选择合适的包装细胞系时，逆转录病毒在人类癌细胞中的转导效率肯定可以提高。此外，本研究表明，Lipofectamine是一种极好的添加剂，可通过病毒进入所特有的病毒包膜蛋白-受体相互作用来提高逆转录病毒的转导效率，而且受体介导的内吞作用似乎不是释放病毒基因组的病毒递送主要途径。
