Ding I, Bruyns E, Li P, Magada D, Paskind M, Rodman L, Seshadri T, Alexander D, Giese T, Schraven B
Immunomodulation Laboratory of the Institute for Immunology University of Heidelberg, Heidelberg, Germany.
Eur J Immunol. 1999 Dec;29(12):3956-61. doi: 10.1002/(SICI)1521-4141(199912)29:12<3956::AID-IMMU3956>3.0.CO;2-G.
The role of the CD45-associated phosphoprotein (LPAP / CD45-AP) during an immune response remains unclear. To understand better the function of LPAP we generated LPAP-deficient mice by disrupting exon 2 of the LPAP gene. LPAP-null mice were healthy and did not show gross abnormalities compared to their wild-type littermates. However, immunofluorescence analysis of T and B lymphocytes revealed a reduced expression of CD45, which did not affect a particular subpopulation. In contrast to a recent report (Matsuda et al., J. Exp. Med. 1998. 187: 1863 - 1870) we neither observed significant alterations of the assembly of the CD45 / lck-complex nor of polyclonal T-cell responses. However, lymphnodes from LPAP-null mice showed increased cellularity, which could indicate that expression of LPAP might be required to prevent expansion of lymphocytes in particular lymphatic organs rather than potentiating immune responses.
CD45相关磷蛋白（LPAP / CD45-AP）在免疫反应中的作用仍不清楚。为了更好地理解LPAP的功能，我们通过破坏LPAP基因的外显子2来培育LPAP缺陷小鼠。与野生型同窝小鼠相比，LPAP基因敲除小鼠健康且未表现出明显异常。然而，对T和B淋巴细胞的免疫荧光分析显示CD45表达降低，这并不影响特定亚群。与最近的一份报告（Matsuda等人，《实验医学杂志》，1998年。187: 1863 - 1870）不同，我们既未观察到CD45 / lck复合物组装的显著改变，也未观察到多克隆T细胞反应的显著改变。然而，LPAP基因敲除小鼠的淋巴结显示细胞增多，这可能表明可能需要LPAP的表达来防止淋巴细胞在特定淋巴器官中扩增，而不是增强免疫反应。
