Meyer O, Hildebrandt M, Schulz B, Blasczyk R, Salama A
Blood Bank, Charité, Campus Virchow-Klinikum, Medical Faculty of Humboldt University of Berlin, Germany.
Transfusion. 1999 Nov-Dec;39(11-12):1256-8. doi: 10.1046/j.1537-2995.1999.39111256.x.
Polymerase chain reaction using sequence-specific primers is widely used for genotyping human platelet antigens (HPA). However, the results of HPA-5 genotyping are still problematic.
New sequence-specific primers were designed for HPA-5 that, together with already published primers, allow simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6. The reliability of the described protocol was determined by using reference DNA samples as well as samples from healthy blood donors.
All primers produced specific amplification products. The genotype and the previously ascertained phenotype of the tested specimens were in concordance in all cases.
The described polymerase chain reaction protocol allows rapid, reliable, simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6.
使用序列特异性引物的聚合酶链反应被广泛用于人类血小板抗原（HPA）基因分型。然而，HPA-5基因分型的结果仍然存在问题。
为HPA-5设计了新的序列特异性引物，这些引物与已发表的引物一起，可对HPA-1、-2、-3、-4、-5和-6进行同时基因分型。通过使用参考DNA样本以及健康献血者的样本确定所描述方案的可靠性。
所有引物均产生特异性扩增产物。在所有情况下，测试样本的基因型与先前确定的表型一致。
所描述的聚合酶链反应方案可对HPA-1、-2、-3、-4、-5和-6进行快速、可靠的同时基因分型。
