Picquart M, Grajcar L, Baron M H, Abedinzadeh Z
Departamento de Física, Universidad Autónoma Metropolitana Iztapalapa, Mexico DF.
Biospectroscopy. 1999;5(6):328-37. doi: 10.1002/(SICI)1520-6343(1999)5:6<328::AID-BSPY2>3.0.CO;2-J.
A spectroscopic study of glutathione (GSH) and glutathione disulfide (GSSG) has been performed using Fourier-transformed infrared absorption and Raman scattering in order to pinpoint the sites of complexation of these two species with water and particularly with H2O2. Molecules of GSH and GSSG were studied in KBr pellets, and in aqueous solutions of H2O, D2O, and H2O with H2O2 (1 mol L(-1)) to characterize the specific influence of the solvent molecules. A time-resolved Raman study was performed for GSH/H2O2, in aqueous solution at 1:1 molar ratio in order to observe the formation of GSSG and to discuss the mechanism of this redox reaction.
为了确定谷胱甘肽（GSH）和谷胱甘肽二硫化物（GSSG）与水特别是与过氧化氢的络合位点，利用傅里叶变换红外吸收和拉曼散射对它们进行了光谱研究。在溴化钾压片中以及在H2O、D2O和含有过氧化氢（1 mol L(-1)）的H2O水溶液中研究了GSH和GSSG分子，以表征溶剂分子的特定影响。对摩尔比为1:1的水溶液中的GSH/过氧化氢进行了时间分辨拉曼研究，以观察GSSG的形成并讨论该氧化还原反应的机制。
