Zimmermann S, Sentenac H
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, D-14424, Germany.
Curr Opin Plant Biol. 1999 Dec;2(6):477-82. doi: 10.1016/s1369-5266(99)00020-5.
Progress in identification of plant ion channels and development of electrophysiological analyses in heterologous expression systems and in planta, in combination with reverse genetic approaches, are providing the possibility of associating molecular entities with physiological functions. Recently, the first attempts to determine in vivo functions using knockout mutants demonstrated the roles of root ion channels. The search for proteins interacting with such channels leads to an even more complex view of the concerted action in protein networks.
在植物离子通道的鉴定以及异源表达系统和植物体内电生理分析技术的发展方面所取得的进展，与反向遗传学方法相结合，为将分子实体与生理功能联系起来提供了可能。最近，利用基因敲除突变体确定体内功能的首次尝试证明了根离子通道的作用。对与这类通道相互作用的蛋白质的研究，使我们对蛋白质网络中的协同作用有了更为复杂的认识。
