Abd El All H, Rye A, Duvillard P
Faculty of Medicine, Suez Canal University, Department of Pathology, Ismailia, Egypt.
Pathol Oncol Res. 1999;5(4):280-4. doi: 10.1053/paor.1999.0134.
Data concerning the expression of p53 in cervical carcinoma, one of the leading cause of death in developing countries, are still confusing. This study was designed to identify p53 in Egyptian cervical carcinoma in an attempt to evaluate its prognostic significance. Eleven chronic cervicitis and 38 invasive carcinoma (31 squamous cell carcinoma (sqcc) and 7 adenocarcinoma, ranging from stage IB to IVB), were stained with the monoclonal antibody anti p53, DO7, using the microwave for antigen retrieval. No immunoreactivity was detected in chronic cervicitis, while nuclear p53 reactivity was detected in all carcinoma and in squamous intra-epithelial lesions (SIL) overlying 8 sqcc. P53 immunohistochemical (IHC) expression was more pronounced in early clinical stages (p=0.007) and in adenocarcinoma compared to sqcc (p=0.015). A positive correlation was present between p53 and heat shock protein 70 (hsp70) expressions (p=0.005). No correlation could be found between p53 expression and tumor infiltrating lymphocytes, the presence or absence of either schistosomiasis or HPV infections. It can be concluded, that in the Egyptian population, p53 immunoreactivity appears to be an early event in cervical neoplasm, and seems to play an important role together with other cell regulatory proteins in the process of carcinogenesis, which could be different between sqcc and adenocarcinoma.
在发展中国家，宫颈癌是主要死因之一，关于其p53表达的数据仍不明确。本研究旨在鉴定埃及宫颈癌中的p53，以评估其预后意义。对11例慢性宫颈炎和38例浸润癌（31例鳞状细胞癌（sqcc）和7例腺癌，分期从IB到IVB），使用微波进行抗原修复，用抗p53单克隆抗体DO7进行染色。在慢性宫颈炎中未检测到免疫反应性，而在所有癌以及8例sqcc上方的鳞状上皮内病变（SIL）中均检测到核p53反应性。与sqcc相比，p53免疫组化（IHC）表达在临床早期（p = 0.007）和腺癌中更明显（p = 0.015）。p53与热休克蛋白70（hsp70）表达之间存在正相关（p = 0.005）。在p53表达与肿瘤浸润淋巴细胞、血吸虫病或HPV感染的有无之间未发现相关性。可以得出结论，在埃及人群中，p53免疫反应性似乎是宫颈肿瘤发生的早期事件，并且在致癌过程中似乎与其他细胞调节蛋白一起发挥重要作用，这在sqcc和腺癌之间可能有所不同。
