通过原位杂交和免疫组织化学法证实大鼠下颌下腺中锰超氧化物歧化酶的表达。

Expression of manganese superoxide dismutase in rat submandibular gland demonstrated by in situ hybridization and immunohistochemistry.

作者信息

Yamamoto H, Sasaki J, Nomura T, Nawa T

机构信息

Department of Oral Anatomy, School of Dentistry, Iwate Medical University, Morioka, Japan.

出版信息

Ann Anat. 1999 Dec;181(6):519-22. doi: 10.1016/s0940-9602(99)80056-9.

DOI:10.1016/s0940-9602(99)80056-9

PMID:10609048

Abstract

Superoxide, an active oxygen species, plays an important role in protecting against bacterial infection. However, it also has adverse effects on health. Manganese superoxide dismutase (Mn-SOD) is a scavenger of superoxide. Antioxygen enzymes such as Mn-SOD exist in various tissues, and provide protections against oxidative injury. We investigated both the expression of Mn-SOD mRNA and the localization of Mn-SOD in adult rat submandibular glands using in situ hybridization and immunohistochemistry. Both Mn-SOD mRNA and Mn-SOD were detected in striated duct cells, and in some granular duct cells and excretory duct cells. With immunoelectron microscopy, many immunolabelings were observed on the mitochondria. These findings suggest that the expression of Mn-SOD mRNA and the localization of Mn-SOD in submandibular glands correlate with the number of mitochondria in cells.

摘要

超氧化物作为一种活性氧，在抵御细菌感染方面发挥着重要作用。然而，它对健康也有不利影响。锰超氧化物歧化酶（Mn-SOD）是超氧化物的清除剂。诸如Mn-SOD之类的抗氧化酶存在于各种组织中，并提供针对氧化损伤的保护作用。我们使用原位杂交和免疫组织化学方法，研究了成年大鼠下颌下腺中Mn-SOD mRNA的表达以及Mn-SOD的定位。在纹状管细胞以及一些颗粒管细胞和排泄管细胞中均检测到了Mn-SOD mRNA和Mn-SOD。通过免疫电子显微镜观察，在线粒体上发现了许多免疫标记。这些发现表明，下颌下腺中Mn-SOD mRNA的表达和Mn-SOD的定位与细胞中的线粒体数量相关。