[竖琴海豹血清中的修饰白蛋白]
[Modified albumin in harp seal blood serum].
作者信息
Erokhina I A
机构信息
Murmansk Marine Biological Institute of Kola Scientific Centre of RAS, Russia.
出版信息
Ukr Biokhim Zh (1999). 1999 Mar-Apr;71(2):75-8.
The content of modified albumin (Am) in harp seal (Pagophilus groenlandica Erxleben, 1777) blood serum was studied. Am was determined by paper electrophoresis by means of re-precipitation in the trichloroacetic acid-ethanol system. Modified albumin content in normal seal pups' blood serum increased from 1990 to 1994. The Am level in undernourished pups was stable from year to year and higher than in normal pups. In oceanarium investigations it was revealed a low albumin resistance to denaturation and the dependence of Am content on the animals' physiological state. Thus there is a possibility to regard modified albumin content as one of the significant parameters in biomonitoring of harp seal population and, moreover, as a supplementary criterion for estimation of seals' health state in captivity.
对格陵兰海豹（Pagophilus groenlandica Erxleben，1777）血清中修饰白蛋白（Am）的含量进行了研究。通过在三氯乙酸 - 乙醇体系中再沉淀，采用纸电泳法测定Am。正常海豹幼崽血清中修饰白蛋白的含量在1990年至1994年期间有所增加。营养不良幼崽的Am水平逐年稳定，且高于正常幼崽。在水族馆调查中发现，白蛋白对变性的抵抗力较低，且Am含量取决于动物的生理状态。因此，有可能将修饰白蛋白含量视为竖琴海豹种群生物监测的重要参数之一，此外，还可作为评估圈养海豹健康状况的补充标准。
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