Peres C M, Van Aken B, Naveau H, Agathos S N
Unit of Bioengineering, Catholic University of Louvain, Belgium.
Appl Microbiol Biotechnol. 1999 Sep;52(3):440-5. doi: 10.1007/s002530051544.
Although isolated on 4-aminobenzoate, Burkholderia cepacia strain PB4 is also able to grow on 4-nitrobenzoate. Degradation of an equimolar mixture of the nitroaromatic compound 4-nitrobenzoate and its corresponding aminoaromatic derivative 4-aminobenzoate by this strain was investigated. Batch experiments showed that, irrespective of preculturing conditions, both compounds were degraded simultaneously. The mixture-degrading ability of B. cepacia strain PB4 was subsequently tested in continuous packed bed reactors (PBR) with the strain immobilized on Celite grade R-633 or R-635. Higher degradation rates were achieved with the larger particles of Celite R-635. Maximum simultaneous degradation rates per liter of packed bed of 0.925 mmol 1(-1) h(-1) 4-nitrobenzoate and 4-aminobenzoate were obtained for an applied loading rate of the same value (0.925 mmol 1(-1) h(-1) of each compound). Even when the applied load was not removed in its entirety, neither of the two compounds was degraded preferentially but a percentage of both of them was mineralized. The present study shows the possibility for a pure strain to biodegrade not only a nitroaromatic compound (4-nitrobenzoate) but also its corresponding amino derivative (4-aminobenzoate) continuously and simultaneously.
尽管洋葱伯克霍尔德氏菌PB4菌株是在4-氨基苯甲酸盐上分离得到的，但它也能够在4-硝基苯甲酸盐上生长。研究了该菌株对硝基芳香化合物4-硝基苯甲酸盐及其相应的氨基芳香衍生物4-氨基苯甲酸盐的等摩尔混合物的降解情况。分批实验表明，无论预培养条件如何，两种化合物都能同时被降解。随后，将洋葱伯克霍尔德氏菌PB4菌株固定在硅藻土R-633或R-635上，在连续填充床反应器（PBR）中测试其混合降解能力。使用较大颗粒的硅藻土R-635可实现更高的降解率。对于相同的施加负荷率（每种化合物0.925 mmol·l⁻¹·h⁻¹），每升填充床获得的4-硝基苯甲酸盐和4-氨基苯甲酸盐的最大同时降解率为0.925 mmol·l⁻¹·h⁻¹。即使施加的负荷没有完全去除，两种化合物也不会被优先降解，而是它们中的一部分被矿化。本研究表明，一种纯菌株有可能连续同时生物降解硝基芳香化合物（4-硝基苯甲酸盐）及其相应的氨基衍生物（4-氨基苯甲酸盐）。
