Bagchi A, Bhattacharya A, Bhattacharya S
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Int J Parasitol. 1999 Nov;29(11):1775-83. doi: 10.1016/s0020-7519(99)00125-3.
A number of small circular DNAs constitute a part of the genome of Entamoeba histolytica. Among them, the 24.5 kb circular DNA encoding rRNA (EhR1) is the most abundant. Pulsed field gel electrophoresis was used to determine if a chromosomal copy of EhR1 exists and what fraction of the total genome is circular. The results show that the chromosomes of E. histolytica are linear, and that no copy of EhR1 could be detected in any of the linear chromosomes.
许多小的环状DNA构成了溶组织内阿米巴基因组的一部分。其中,编码rRNA的24.5 kb环状DNA(EhR1)最为丰富。采用脉冲场凝胶电泳来确定EhR1是否存在染色体拷贝以及环状DNA在整个基因组中所占的比例。结果显示,溶组织内阿米巴的染色体是线性的,并且在任何一条线性染色体中均未检测到EhR1的拷贝。