Rae J L, Shepard A R
Department of Physiology & Biophysics, Department of Ophthalmology, Mayo Foundation, Rochester, Minnesota 55905, USA.
Curr Eye Res. 2000 Feb;20(2):144-52.
To determine the existence of inward rectifier (Kir2.1) sequences in mRNA from corneal epithelium and endothelium.
cDNA library construction, cloning, PCR, patch clamp. RESULTS. Both the corneal epithelium and endothelium contain mRNA for Kir2.1 inwardly rectifying potassium channels. When the cDNA is transfected into Chinese hamster ovary cells, the channel currents match those expected from Kir2.1 inward rectifiers.
The mRNA for Kir2.1 potassium channels exists in corneal epithelium and endothelium. Therefore, Kir2.1 inwardly rectifying potassium channels probably make some contributions to the resting voltages of cornea epithelium and endothelium. Previous data, however, suggest that they are probably not the dominant contributors in these preparations.
确定角膜上皮和内皮细胞mRNA中内向整流器（Kir2.1）序列的存在情况。
cDNA文库构建、克隆、聚合酶链反应、膜片钳技术。结果：角膜上皮和内皮细胞均含有Kir2.1内向整流钾通道的mRNA。当将该cDNA转染到中国仓鼠卵巢细胞中时，通道电流与Kir2.1内向整流器预期的电流相匹配。
Kir2.1钾通道的mRNA存在于角膜上皮和内皮细胞中。因此，Kir2.1内向整流钾通道可能对角膜上皮和内皮细胞的静息电压有一定贡献。然而，先前的数据表明，它们可能不是这些制剂中的主要贡献者。
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