Gaze W H, Wootten R
Institute of Aquaculture, University of Stirling, UK.
Syst Parasitol. 1999 Jul;43(3):167-74. doi: 10.1023/a:1006149318264.
Specimens of Trichodina domerguei Wallengren, 1897, T. intermedia (Lom, 1961) and Paratrichodina incissa (Lom, 1959) were sonicated to liberate skeletal components of the adhesive disc. This enabled SEM observation of the taxonomically important structures obscured in preparations of complete cells. A previously undescribed peg-like structure on the centrifugal surface of the central part of the denticles is revealed in T. domerguei. In P. incissa the ray apophysis and its supporting apophysis appear to be absent, providing an additional characteristic to discriminate it from species of the genus Trichondina Ehrenberg, 1838. From silver stained and SEM preparations of T. intermedia and P. incissa important differences in denticle blade form are apparent, underlining the value of observation of isolated skeletal structures by electron microscopy.
1897年的多氏车轮虫(Trichodina domerguei Wallengren)、1961年的中间车轮虫(T. intermedia (Lom))和1959年的刻纹拟车轮虫(Paratrichodina incissa (Lom))的标本经超声处理以释放附着盘的骨骼成分。这使得能够通过扫描电子显微镜观察完整细胞制备物中被掩盖的具有分类学重要性的结构。在多氏车轮虫中发现了齿体中央部分离心表面上一个先前未描述的钉状结构。在刻纹拟车轮虫中,辐状突及其支撑突似乎不存在,这为将其与1838年埃伦贝格(Ehrenberg)的车轮虫属(Trichondina)物种区分开来提供了一个额外的特征。从中间车轮虫和刻纹拟车轮虫的银染和扫描电子显微镜制备物中可以明显看出齿体叶片形态的重要差异,这突出了通过电子显微镜观察分离的骨骼结构的价值。