酸性磷脂双分子层微囊：一种多功能的膜模型系统。
Acidic phospholipid bicelles: a versatile model membrane system.
作者信息
Struppe J, Whiles J A, Vold R R
机构信息
Department of Chemistry, University of California San Diego, La Jolla, California 92093-0359 USA.
出版信息
Biophys J. 2000 Jan;78(1):281-9. doi: 10.1016/S0006-3495(00)76591-X.
Abstract
With the aim of establishing acidic bicellar solutions as a useful membrane model system, we have used deuterium NMR spectroscopy to investigate the properties of dimyristoyl/dihexanoylphosphatidylcholine (DMPC/DHPC) bicelles containing 25% (w/w in H(2)O) of either dimyristoylphosphatidylserine (DMPS) or dimyristoylphosphatidylglycerol (DMPG). The addition of the acidic lipid component to this lyotropic liquid crystalline system reduces its range of stability because of poor miscibility of the two dimyristoylated phospholipids. Compared to the neutral bicelles, which are stable at pH 4 to pH 7, acidic bicelles are stable only from pH 5.5 to pH 7. Solid-state deuterium NMR analysis of d(54)-DMPC showed similar ordering in neutral and acidic bicelles. Fully deuterated DMPS or DMPG is ordered in a way similar to that of DMPC. Study of the binding of the myristoylated N-terminal 14-residue peptide mu-GSSKSKPKDPSQRR from pp60(nu-src) to both neutral and acidic bicelles shows the utility of these novel membrane mimetics.
摘要
为了将酸性双分子层溶液确立为一种有用的膜模型系统，我们使用氘核磁共振光谱法研究了含有25%（水相中重量比）二肉豆蔻酰磷脂酰丝氨酸（DMPS）或二肉豆蔻酰磷脂酰甘油（DMPG）的二肉豆蔻酰/二己酰磷脂酰胆碱（DMPC/DHPC）双分子层的性质。由于两种二肉豆蔻酰化磷脂的互溶性较差，向这种溶致液晶系统中添加酸性脂质成分会降低其稳定性范围。与在pH 4至pH 7稳定的中性双分子层相比，酸性双分子层仅在pH 5.5至pH 7稳定。d(54)-DMPC的固态氘核磁共振分析表明，中性和酸性双分子层中的有序性相似。全氘代的DMPS或DMPG的有序排列方式与DMPC相似。对来自pp60(nu-src)的肉豆蔻酰化N端14残基肽mu-GSSKSKPKDPSQRR与中性和酸性双分子层结合的研究表明了这些新型膜模拟物的实用性。
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