电压钳制下心室肌细胞中肌浆网钙泵的反向模式及负荷依赖性胞质钙下降
Reverse mode of the sarcoplasmic reticulum calcium pump and load-dependent cytosolic calcium decline in voltage-clamped cardiac ventricular myocytes.
作者信息
Shannon T R, Ginsburg K S, Bers D M
机构信息
Department of Physiology, Loyola University Chicago, Maywood, Illinois, 60153, USA.
出版信息
Biophys J. 2000 Jan;78(1):322-33. doi: 10.1016/S0006-3495(00)76595-7.
Abstract
We have characterized Ca decline in voltage-clamped rabbit ventricular myocytes with progressive increases in sarcoplasmic reticulum (SR) calcium load. "Backflux" through the SR calcium pump is a critical feature which allows realistically small values for SR calcium leak fluxes to be used. Total cytosolic calcium was calculated from the latter part of Ca decline using rate constants for cellular calcium buffers. Intra-SR calcium buffering characteristics were also deduced. We found that the net SR calcium pump flux and rate of Ca decline decreased as the SR free [Ca] rose, with pump parameters held constant. We have therefore characterized for the first time in intact myocytes both forward and reverse SR calcium pump kinetics as well as intra-SR calcium buffering and SR calcium leak. We conclude that the reverse flux through the SR calcium pump is an important factor in comprehensive understanding of dynamic SR calcium fluxes.
摘要
我们已对电压钳制的兔心室肌细胞中[Ca]（i）的下降进行了表征，随着肌浆网（SR）钙负荷的逐渐增加。通过SR钙泵的“回流”是一个关键特征，它允许使用实际较小的SR钙漏通量值。利用细胞钙缓冲剂的速率常数，从[Ca]（i）下降的后半部分计算总胞质钙。还推导了SR内钙缓冲特性。我们发现，在泵参数保持不变的情况下，随着SR游离[Ca]的升高，SR钙泵的净通量和[Ca]（i）下降速率降低。因此，我们首次在完整的心肌细胞中表征了正向和反向SR钙泵动力学以及SR内钙缓冲和SR钙泄漏。我们得出结论，通过SR钙泵的反向通量是全面理解动态SR钙通量的一个重要因素。
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