Evans C S, Startin J R, Goodall D M, Keely B J
Chemistry Department, University of York, Heslington, York YO10 5DD, UK.
Rapid Commun Mass Spectrom. 2000;14(2):112-7. doi: 10.1002/(SICI)1097-0231(20000130)14:2<112::AID-RCM841>3.0.CO;2-0.
Optimisation of the activation parameters for ion trap mass spectrometric analysis of the chlormequat cation using simplex optimisation enabled the product ion (m/z 58) response to be improved 1000-fold. A comparison of the sensitivity of the optimised ion trap mass spectrometer with that of a triple quadrupole mass spectrometer for liquid chromatography/tandem mass spectrometry (LC/MS/MS) showed that similar limits of detection (LODs) could be achieved. For the MS/MS transition of the (35)Cl precursor to the most abundant product, LODs were 0.8 ng cation mL(-1) (0.004 mg cation kg(-1) pear equivalent) and 1.0 ng cation mL(-1) (0.005 mg cation kg(-1) pear equivalent) on the triple quadrupole and ion trap instrument, respectively.
使用单纯形优化法对矮壮素阳离子进行离子阱质谱分析时，对激活参数进行优化可使产物离子（m/z 58）的响应提高1000倍。将优化后的离子阱质谱仪与用于液相色谱/串联质谱（LC/MS/MS）的三重四极杆质谱仪的灵敏度进行比较，结果表明可以实现相似的检测限（LOD）。对于（35）Cl前体到最丰富产物的MS/MS转换，三重四极杆仪器和离子阱仪器的检测限分别为0.8 ng阳离子 mL⁻¹（0.004 mg阳离子 kg⁻¹梨当量）和1.0 ng阳离子 mL⁻¹（0.005 mg阳离子 kg⁻¹梨当量）。
