Iida Y, Yonemura H, Oh K B, Saito M, Matsuoka H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Yakugaku Zasshi. 1999 Dec;119(12):964-71. doi: 10.1248/yakushi1947.119.12_964.
Antifungal activity of medicinal plants against Aspergillus niger was evaluated using Bio-Cell Tracer (BCT). By this system enabled the real time determination of hyphal growth rate could be done in the presence or absence of Chinese herbal extracts. Of the 41 herbal extracts with 60% acetone extracts tested by the present method, 26 were found to contain active components against A. niger hyphal growth. In contrast, by conventional methods, no active component was detected from every herbal extract except 3 herbal extracts. From the extract of Anemarrhena asphodeloides, one of active components was isolated and its structure determined by NMR, UV, and mass spectroscopy. The compound was identified as broussonin A (2-3-(4-hydroxyphenyl)propyl)-5-methoxy-phenol) which was formerly reported as a phytoalexin of Broussonetia papyrifera Vent.
使用生物细胞示踪剂（BCT）评估药用植物对黑曲霉的抗真菌活性。通过该系统能够在有或没有中草药提取物的情况下实时测定菌丝生长速率。用本方法测试的41种含60%丙酮提取物的草药提取物中，发现26种含有抑制黑曲霉菌丝生长的活性成分。相比之下，通过传统方法，除了3种草药提取物外，未从每种草药提取物中检测到活性成分。从知母提取物中分离出一种活性成分，并通过核磁共振、紫外和质谱确定其结构。该化合物被鉴定为布罗索宁A（2-3-（4-羟基苯基）丙基）-5-甲氧基苯酚），以前报道为构树的一种植物抗毒素。
