衰老过程中大鼠比目鱼肌中与性别和甲状腺激素相关的必需肌球蛋白轻链同工型表达的转变
Gender- and thyroid hormone-related transitions of essential myosin light chain isoform expression in rat soleus muscle during ageing.
作者信息
Biral D, Ballarin F, Toscano I, Salviati G, Yu F, Larsson L, Betto R
机构信息
National Research Council Unit for Muscle Biology and Physiopathology, c/o Department of Biomedical Sciences, University of Padova, Italy.
出版信息
Acta Physiol Scand. 1999 Dec;167(4):317-23. doi: 10.1046/j.1365-201x.1999.00621.x.
In this brief review, the modulatory influence of essential myosin light chain (MLC) isoforms on muscle cell contractility is discussed. Specific interest is focused on the expression of the MLC1Sa and MLC1Sb isoforms in the slow-twitch soleus muscle in male and female rats, during ageing and after thyroid hormone treatment. According to two-dimensional gel electrophoresis analysis, the MLC1Sa/MLC1SB ratio increased during ageing in both males and females in parallel with the age-related decrease in shortening velocity reported in muscle fibres expressing the slow (type 1) myosin heavy chain (MHC) isoform. However, the MLC1Sa and MLC1Sb isoform expression responded to thyroid hormone treatment in a complex manner which did not parallel the age-related changes in shortening velocity reported in hyperthyroid animals. Thus, if MLC1Sa and MLC1Sb isoforms modulate shortening velocity in type 1 fibres, then other modulators of shortening velocity are not regulated by thyroid hormone in co-ordination with these essential MLCs.
在这篇简短的综述中，我们讨论了必需肌球蛋白轻链（MLC）同工型对肌肉细胞收缩性的调节作用。特别关注MLC1Sa和MLC1Sb同工型在雄性和雌性大鼠慢肌比目鱼肌中的表达，以及衰老过程中和甲状腺激素处理后的表达情况。根据二维凝胶电泳分析，在雄性和雌性大鼠衰老过程中，MLC1Sa/MLC1SB比值均升高，这与表达慢肌（1型）肌球蛋白重链（MHC）同工型的肌纤维中与年龄相关的缩短速度下降相一致。然而，MLC1Sa和MLC1Sb同工型的表达对甲状腺激素处理的反应较为复杂，这与甲状腺功能亢进动物中报道的与年龄相关的缩短速度变化并不平行。因此，如果MLC1Sa和MLC1Sb同工型调节1型纤维的缩短速度，那么缩短速度的其他调节因子并不与这些必需的MLC协同受甲状腺激素调节。
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