荚膜红细菌regB基因DNA序列的校正及其对传感激酶regB膜拓扑结构的影响
Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase regB.
作者信息
Chen W, Jäger A, Klug G
机构信息
Institut für Mikrobiologie und Molekularbiologie, D-35392 Giessen, Germany.
出版信息
J Bacteriol. 2000 Feb;182(3):818-20. doi: 10.1128/JB.182.3.818-820.2000.
Abstract
We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.
摘要
我们校正了先前发表的荚膜红细菌中编码组氨酸传感器激酶的regB基因序列。推导的RegB氨基酸序列在N端有一个额外的推定跨膜结构域。对RegB-PhoA和RegB-LacZ融合蛋白的分析支持了RegB具有六个跨膜结构域的拓扑模型。
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