瑟氏泰勒虫（韩国分离株）主要梨形虫蛋白（p33）的表达及其在豚鼠中的免疫原性。

Expression of major piroplasm protein (p33) of Theileria sergenti (Korean isolate) and its immunogenicity in guinea pigs.

作者信息

Kang S W, Kweon C H, Choi E J, Yoon Y D

机构信息

National Veterinary Research and Quarantine Service, MAF, Anyang, Korea.

出版信息

Korean J Parasitol. 1999 Dec;37(4):277-83. doi: 10.3347/kjp.1999.37.4.277.

DOI:10.3347/kjp.1999.37.4.277

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2733206/

Abstract

To investigate the development of a subunit vaccine against theileriosis in cattle, the DNA fragments encoding piroplasm surface protein (p33) of Theileria sergenti of a Korean isolate were expressed in baculoviruses. The expressed p33 was characterized by indirect fluorescent antibody (IFA) and western blotting analysis. The expression of p33 was mainly detected on the surface of infected Sf21 cells by IFA. The immunoblotting analysis revealed the presence of a same molecular weight protein band of p33. The antigenicity of expressed polypeptide was further examined through the inoculation of a guinea pig. The sera of guinea pigs immunized with p33 expressed cell lysate showed similar fluorescent antibody patterns and reacted with the same molecular weight protein of T. sergenti in immunoblotting analysis, thus indicating that this protein can be a promising candidate for a subunit vaccine in the future.

摘要

为研究牛泰勒虫病亚单位疫苗的研发，将编码韩国分离株瑟氏泰勒虫梨形虫表面蛋白（p33）的DNA片段在杆状病毒中进行表达。通过间接荧光抗体（IFA）和蛋白质免疫印迹分析对表达的p33进行表征。IFA主要在感染的Sf21细胞表面检测到p33的表达。免疫印迹分析显示存在一条分子量相同的p33蛋白条带。通过接种豚鼠进一步检测表达多肽的抗原性。用p33表达细胞裂解物免疫的豚鼠血清在免疫印迹分析中显示出相似的荧光抗体模式，并与瑟氏泰勒虫分子量相同的蛋白发生反应，因此表明该蛋白未来可能是一种有前景的亚单位疫苗候选物。

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