Olson A J, Picones A, Julian D, Korenbrot J I
Department of Physiology, School of Medicine, University of California at San Francisco, 94143, USA.
J Neurosci Methods. 1999 Nov 15;93(2):91-100. doi: 10.1016/s0165-0270(99)00111-9.
The retina in teleost fish continues to grow throughout much of the life of the animal, in part by the continuing differentiation of new tissue at the retinal margin, an area termed the peripheral growth zone (PGZ) (Lyall, Q J Micros Sci, 1957:98:101-110). We have developed a retinal slice preparation--including the PGZ--from juvenile rainbow trout (Onchorynchus mykiss), a species in which retinal growth is rapid and the PGZ is correspondingly pronounced. The PGZ slice preparation contains a time line of retinal development, with cells at different stages of maturation present side by side. We present evidence that the birth sequence of the various retinal cell types in the PGZ recapitulates the sequence during embryonic development. We also report data on the rate of growth of the PGZ in juvenile trout in vivo. Finally, we have used the PGZ slice preparation to make whole-cell voltage clamp recordings from individual retinal GCs at both early and late stages of maturation. We report that the amplitude of delayed rectifier and A-type potassium currents increases during GC maturation.
硬骨鱼的视网膜在动物的大部分生命过程中持续生长，部分原因是视网膜边缘新组织的持续分化，该区域称为外周生长区（PGZ）（莱尔，《微观科学季刊》，1957年：98：101 - 110）。我们已经从幼年虹鳟鱼（Onchorynchus mykiss）中开发出一种包括PGZ的视网膜切片标本，虹鳟鱼是一种视网膜生长迅速且PGZ相应明显的物种。PGZ切片标本包含视网膜发育的时间线，不同成熟阶段的细胞并列存在。我们提供的证据表明，PGZ中各种视网膜细胞类型的产生顺序重现了胚胎发育过程中的顺序。我们还报告了幼年虹鳟鱼体内PGZ的生长速率数据。最后，我们使用PGZ切片标本对成熟早期和晚期的单个视网膜神经节细胞进行全细胞电压钳记录。我们报告说，延迟整流钾电流和A型钾电流的幅度在神经节细胞成熟过程中增加。
