Karp J E, Burke P J
Cancer Res. 1976 May;36(5):1674-9.
Factors that influence the proliferation of marrow elements can be detected in sera. To determine the function and to compare the effect of these factors, cells were obtained from patients with normal and leukemic bone marrows. The effects of drug-induced stimulatory and inhibitory sera and leukemic pretreatment sera over time (0 to 6 days) on proliferation and granulocytic morphology of normal and leukemic bone marrow cells in culture were evaluated. Increased proliferation was associated with stimulatory sera, while inhibitory and leukemic pretreatment sera retarded proliferation of both normal and malignant cells. Exposure of normal proliferative cells to inhibitory or leukemic pretreatment sera yielded the greatest increase in mature granulocyte forms. In contrast, although the proliferative response of leukemic cells to these sera was similar to normal, maturation was minimal. These data suggest that leukemic pretreatment sera are similar to inhibitory sera and are not leukemogenic. Both retard proliferation of normal and leukemic bone marrow cells while enhancing maturation of normal cells. Leukemic myeloblasts, however, cannot be made to mature by these humoral regulators.
影响骨髓细胞增殖的因素可在血清中检测到。为了确定这些因素的功能并比较其作用，从正常骨髓和白血病骨髓患者中获取细胞。评估了药物诱导的刺激血清和抑制血清以及白血病预处理血清在0至6天内对培养的正常和白血病骨髓细胞增殖及粒细胞形态的影响。增殖增加与刺激血清有关，而抑制血清和白血病预处理血清则抑制正常细胞和恶性细胞的增殖。正常增殖细胞暴露于抑制血清或白血病预处理血清会使成熟粒细胞形态增加最多。相比之下，尽管白血病细胞对这些血清的增殖反应与正常细胞相似，但成熟程度最低。这些数据表明，白血病预处理血清与抑制血清相似，不具有致白血病性。两者都抑制正常和白血病骨髓细胞的增殖，同时促进正常细胞的成熟。然而，白血病原始粒细胞不能通过这些体液调节因子实现成熟。
